urn:osa:lingual.bio:rec:1edabb12-d315-4cb2-9b09-679eec298b66@1Bulk RNAseq of BMDM-T cells co-culture system with or without tumor antigen and IL-1beta or IL-18
Expression profiling by high throughput sequencingSummary
To investigate the transcriptome changes of BMDM-T cell co-culture system, spleen lyphocytes were cultured with WT or Tmem175-/- BMDMs in 1640 supplied with FBS, m-CSF, and IL-2 for 48 hours. Tumor antigens, IL-1beta (500 pg/ml), or IL-18 (500 pg/ml) were added during the co-culture period. At the end of the culture, cells were harvested for RNA seq.
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urn:osa:lingual.bio:rec:1edabb12-d315-4cb2-9b09-679eec298b66@1