Single‑cell RNA sequencing of orthotopic GBM tumors following oncolytic adenovirus treatment

Summary

Mice bearing intracranial GL261‑CAR tumors were treated intratumorally with PBS (control), oAd, or oAd‑mCD47/PD‑L1‑mIL‑12 (1 × 109 pfu). Tumors were harvested three days after the second viral injection and dissociated into single‑cell suspensions using the SeekMate Tissue Dissociation Reagent Kit A Pro (SeekGene, K01801‑30).Single‑cell RNA‑seq libraries were constructed using the SeekOne Digital Droplet Single‑Cell 3′ Library Preparation Kit (SeekGene, #K00202).Single‑cell transcriptomic data demonstrate that oAd‑mCD47/PDL1‑mIL‑12 orchestrates a multi‑faceted immune response: expanding cytotoxic and memory T‑cell subsets, polarizing macrophages toward an antigen‑presenting and chemokine‑secreting phenotype, and enhancing reciprocal signaling between immune and cancer cells to promote tumor clearance.

Details

Organism

Mus musculus

Samples

9 samples

Publication Date

Jan 20, 2026

Platform

34290

Entry Type

GSE

Identifier